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PP9 - IRCCS Burlo - Institute of Child Health IRCCS "Burlo Garofolo" Trieste

Phage display antibodies assay, mucosal immunology, citofluorimetric assay, diagnosis and treatment of autoimmune disorders.


DESCRIPTION OF ORGANIZATION/DEPARTMENT

The Burlo Garofolo Hospital was established on November 18th 1856, when the Spedale Infantile was instituted in order to grant free medical care to poor children. On 1968 the Institute was designated by the Ministry of Health as IRCCS. The Institute has promoted and implemented an innovative health culture, based on innovative policies aimed at reduction of hospital stay and humanization of medical care. The Hospital is the unique health care Institute for Mother and Child Health within the area the sourrounding region.
 


TECHNOLOGIES AND EXPERTISE AVAILABLE

We are developing new methods to make an early diagnosis of celiac disease by using new assays:
  • phage display antibody library (antibodies are cloned in bacterial cells and expressed on phages to be eventually isolated through selection on the antigen tissue transglutaminase)
  • double immunofluorescent staining (investigation of antibodies IgA anti-tissue transglutaminase on criosections obtained from intestinal biopsies)
  • flow cytometric analysis of activated beads (beads activated with tissue transglutaminase are incubated with antibodies extracted from intestinal biopsies to be analysed in flow cytometry)
 
 
 

FIELD OF RESEARCH/ACTIVITIES

Our interests are based on autoimmune disorders. We organised a serum and tissue bio-bank from patients suffering from organ specific autoimmune disorders (e.g. type 1 diabetes, thyroiditis, celiac disease, rheumatoid arthritis) or from other inflammatory diseases (e.g. Crhon disease, eosinophyl-gastritis, ulcerative colitis) with a large samples stored at -80 C° (10000 serum samples and 1500 intestinal biopsies).
Our main goal is to study celiac disease (CD), an autoimmune-mediated enteropathy characterised by gluten-triggered small bowel mucosal lesions in genetically susceptible individuals carrying the CD-related human leukocyte antigen (HLA) DQ2 or DQ8 haplotypes.
 


KEY WORDS

Autoimmunity, inflammatory diseases, celiac disease, autoantibodies, early diagnoses.

 

 
Institute of Child Health IRCCS "Burlo Garofolo" Trieste
  Tarcisio Not
Via dell’Istria 65/1, 34100 TRIESTE Italy
Italy